Laboratory testing for recent nicotine exposure
Measure cotinine, the body’s main nicotine metabolite.
Results usually arrive within five working days.
Book Your Cotinine Test Check whether this test suits youSample
Urine
Typical detection
Around 2–4 days
Results
Usually 5 working days
Attendance
Walk-in after confirmation
The test measures cotinine within a urine sample.
Your body produces cotinine after processing nicotine.
It provides objective evidence of recent nicotine exposure.
Support insurance or underwriting nicotine-testing requirements.
Check recent nicotine exposure during smoking cessation.
Meet requested tobacco-free workplace testing requirements.
Provide documented testing when standard reporting is accepted.
Confirm the required reporting standard before ordering.
Cotinine can follow smoking, vaping or nicotine replacement.
Nicotine pouches and other nicotine products also affect results.
The test cannot distinguish between these nicotine sources.
Choose the option matching your required evidence.
| Test | Sample | Coverage | Common purpose |
|---|---|---|---|
| Urine cotinine test | Urine | Recent nicotine exposure | Insurance, workplace or personal testing |
| Blood cotinine test | Blood | Recent nicotine exposure | When blood testing is requested |
| Chain-of-custody testing | As agreed | Formally documented collection | Court or other formal evidence |
Cotinine is typically detectable for two to four days.
The detection period varies considerably between individuals.
Detection can sometimes extend to around seven days.
Nicotine use, metabolism and collection timing affect detection.
Cotinine is a primary metabolite of nicotine.
It lasts longer than nicotine and supports exposure testing.
Below 50 ng/ml: usually negative or minimal exposure.
50–500 ng/ml: equivocal and may require repeat testing.
Above 500 ng/ml: usually consistent with significant nicotine exposure.
The final laboratory report provides the authoritative interpretation.
It cannot identify smoking, vaping or replacement therapy.
It cannot calculate precisely how much nicotine was used.
Passive nicotine exposure can influence lower results.
A negative result cannot exclude older nicotine exposure.
The Doctors Laboratory
76 Wimpole Street
London W1G 9RT
Monday–Friday: 7am–7pm
Saturday: 7am–1pm
Sunday: 8am–11am
Walk in during opening hours after receiving confirmation.
Bring original photo identification. Phone images and photocopies are unacceptable.
Results usually arrive within five working days.
Collection time and laboratory processing may affect turnaround.
A doctor reviews results before secure email delivery.
Measure cotinine for recent nicotine exposure.
Testing uses UKAS-accredited The Doctors Laboratory.
Receive a measured result with laboratory interpretation.
A doctor reviews reports before secure delivery.
It measures a primary nicotine metabolite within urine.
Yes. Nicotine-containing vaping can produce measurable cotinine.
Yes. Patches, gum and other nicotine products can affect results.
Usually two to four days, sometimes around seven days.
The result cannot confirm positive or negative exposure.
No. Choose chain-of-custody testing for formal evidence requirements.
No. Walk in after receiving your attendance confirmation.
Yes. Bring an original passport or driving licence.
Order online and receive your London attendance confirmation.
Book Your Cotinine TestSince 2015, we’ve provided confidential drug and alcohol testing across London.
We support individuals, employers, solicitors, social workers and other organisations.
Order online, then attend our Central London Patient Reception after confirmation. Selected home-testing options are also available.
Samples are analysed by UKAS-accredited laboratories, including The Doctors Laboratory. Results are reviewed and sent securely by email.
Need help choosing the correct test?
Call 0207 871 3015 or email enquiry@drugtest.london.
Drug Test London is a brand of AIS Health Ltd. Company number: 09835294.